Composite
Part:BBa_K3166057:Design
Designed by: Yijia Ren Group: iGEM19_SEFLS_Shanghai (2019-10-16)
pBBR1MCS-1(p15A)-AtoB-HMGS-tHMGR
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 2374
Illegal PstI site found at 2602 - 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 2374
Illegal PstI site found at 2602 - 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 2374
Illegal PstI site found at 2602 - 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 2374
Illegal PstI site found at 2602
Illegal NgoMIV site found at 181
Illegal NgoMIV site found at 3211
Illegal NgoMIV site found at 3403
Illegal NgoMIV site found at 3570
Illegal NgoMIV site found at 4101
Illegal AgeI site found at 3096 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 235
Illegal SapI.rc site found at 282
Illegal SapI.rc site found at 3657
Design Notes
PCR
Source
pBBR1MCS-1(p15A)-AtoB-HMGS-tHMGR