Composite

Part:BBa_K3166057:Design

Designed by: Yijia Ren   Group: iGEM19_SEFLS_Shanghai   (2019-10-16)


pBBR1MCS-1(p15A)-AtoB-HMGS-tHMGR


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 2374
    Illegal PstI site found at 2602
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 2374
    Illegal PstI site found at 2602
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 2374
    Illegal PstI site found at 2602
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 2374
    Illegal PstI site found at 2602
    Illegal NgoMIV site found at 181
    Illegal NgoMIV site found at 3211
    Illegal NgoMIV site found at 3403
    Illegal NgoMIV site found at 3570
    Illegal NgoMIV site found at 4101
    Illegal AgeI site found at 3096
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 235
    Illegal SapI.rc site found at 282
    Illegal SapI.rc site found at 3657


Design Notes

PCR


Source

pBBR1MCS-1(p15A)-AtoB-HMGS-tHMGR

References